Discovery & Global Proteomics
Unbiased, Deep Mass-Spectrometry Profiling
Our B2B Discovery Proteomics Service utilizes nano-liquid chromatography tandem mass spectrometry (LC-MS/MS) to isolate, identify, and quantify more than 8,000 distinct expressed proteins in a single sample. We deliver complete, unbiased expression maps across cell lines, patient tissues, organoids, and clinical biofluids to isolate predictive trial biomarker signatures.
Bypassing Targeted Biomarker Blind Spots
Traditional preclinical discovery relies on targeted assay panels (such as Western Blots or ELISAs) that measure a restricted selection of 50 to 200 predefined proteins. However, complex oncological mechanisms and drug resistance cascades regularly activate pathways outside these narrow ranges, creating critical biological blind spots.
By utilizing high-resolution mass spectrometers (such as Orbitrap and Q-TOF systems), Yatiri Bio's global discovery platform captures the complete proteomic state. This allows researchers to monitor protein-level responses to therapeutic candidate molecules, characterizing downstream metabolic pathways and off-target signaling events across the complete proteome.
Technical Workflow & Sample Specification
To maintain high quantitative accuracy and reproducible profiles, Yatiri Bio incorporates rigorous chemical isotope labeling (such as Tandem Mass Tagging - TMT) and label-free quantification (LFQ) workflows:
| Matrix Type | Min. Required Input | Typical Processing Time |
|---|---|---|
| Cultured Cells / Organoids | 1x10⁶ Cells | 10 Business Days |
| Fresh Frozen Tissue | 15 mg Tissue | 14 Business Days |
| Blood Plasma / Serum | 20 μL Sample | 14 Business Days |
| Isolated Cell Lysate | 50 μg Protein | 7 Business Days |
All sample preparations are performed inside our San Diego laboratory facility. Following protein extraction, cell lysis, and trypsin digestion, peptide fragments are separated via nano-LC before injection. Deep sequence analysis is executed via high-resolution MS/MS to determine sequence identifiers and peptide spectral counts.
Discovery Proteomics Buyer Questions
When should a team use discovery proteomics?
Use discovery proteomics when the protein markers, resistance pathways, or responder subsets are not known in advance and targeted panels would be too narrow.
What does Yatiri Bio return?
Typical outputs include quantified protein tables, pathway-level interpretation, and candidate biomarker signatures that can feed ProteoModels, AI analysis, or CLIA/CAP assay development.
Request Project Quote
Discovery proteomics campaigns are tailored to study design parameters (sample count, matrix type, multiplex requirement).